One label, one tube, Sanger DNA sequencing in one and two lanes on a gel

One label, one tube, Sanger DNA sequencing in one and two lanes on a gel.

Sequencing in less than four lanes on a gel, using only one fluorescent label for the four bases (without variations in electrophoretic mobilities or problems with spectral overlap) in one tube was introduced in (1). In that process T7 DNA polymerase and buffer containing magnesium ions were used. By adjusting the ddNTPs ratios the sequence is deduced from the peak magnitudes corresponding to t...

Part One Sanger DNA Sequencing

Queueing Inventory System in a Two-level Supply Chain with One-for-One Ordering Policy

Consider a two-level inventory system consisting of one supplier and one retailer. The retailer faces a Poisson demand with a known rate and applies base stock (one-for-one ordering) policy. That is, his inventory position is set to a pre-determined level, so the demand pattern is transferred exactly to the supplier. The supplier has an inventory system and a service unit with exponentially dis...

One verse, One Concept

No Abstract

One verse, One Concept

No Abstract